Calibration of Fourier Transform Infra-red Spectrophotometer (FTIR) SOP

FTIR Calibration SOP | Calibration of FTIR | IR Calibration | Calibration of Infrared Spectrophotometer |

  • FTIR Calibration SOP covers below points:
    • Precautions
    • Calibration Parameters of FTIR
      • Verification of Wave Number Scale
      • Control of Resolution Performance
    • FTIR Calibration format
1.0 OBJECTIVE :
  • To provide a procedure for Calibration of Fourier Transform Infra-Red (FTIR) Spectrophotometer.
2.0 SCOPE :
  • This procedure is applicable to FTIR Spectrophotometer
3.0 RESPONSIBILITY :
  • Quality Control Chemist and above
4.0 ACCOUNTABILITY:
  • Quality Control Head
5.0 PROCEDURE :

5.1 PRECAUTIONS

  • Place the instrument as far as possible away from any strong magnetic or electric field or any electrical appliances generating a high frequency.
  • Avoid placing it, where severe vibrations exist.
  • Place the instrument free from dust and corrosive gases.

5.2 CALIBRATION

  • Change the assembly for calibration instead of assembly for analysis for the calibration.
  • Handle the calibration assembly very carefully.
  • Handle the polystyrene film very gently and avoid direct contact to the film.
  • Verification of Wave Number Scale
    • Measure the spectrum of polystyrene film 4000 cm-1 to 650 cm-1 after the routine Background correction.
    • Following bands should appear at the frequencies indicated below and should not deviate from the designated frequency by more than the tolerance indicated.  
Sr. No.Wave-number (cm-1)Acceptable Tolerance (cm-1)
01.3060.0    ± 1.03059.0 –3061.0
02.2849.5    ± 1.02848.5 – 2850.5
03.1942.9    ± 1.01941.9 – 1943.9
04.1601.2    ± 1.01600.2 – 1602.2
05.1583.0    ±  1.01582.0 –1584.0
06.1154.5     ± 1.01153.5 – 1155.5
07.1028.3    ±  1.01027.3 – 1029.3

  • Control of Resolution Performance
    • Expand the recorded polystyrene spectrum in the region 3200 cm-1 to   2600 cm-1.
    • Note down the absorption at wave numbers 2870 cm-1 (tolerance ± 1 cm-1) and 2849.5 cm-1  (tolerance ± 1 cm-1).  
    • The difference between the absorbances at the absorption minimum at 2870 cm-1 and the absorption maximum at 2849.5 cm-1 is greater than 0.33. 
    • Expand the polystyrene spectrum in the wave number region 1800 cm-1 to 1400 cm-1.
    • Note down the absorption at wave numbers the difference the absorption  at 1583 cm-1 (tolerance ± 1 cm-1) and 1589 cm-1 (tolerance ± 1 cm-1).
    • The difference between the absorbances at the absorption minimum at 1589 cm-1 and the absorption maximum at 1583 cm-1 is greater than 0.08.
Control of Resolution Performance Tolerance Limit


For General information on Infrared Absorption Spectroscopy click link: INFRARED ABSORPTION SPECTROSCOPY


 6.0 ABBREVIATIONS:
AbbreviationExpanded form
FTIRFourier Transform Infra-Red
LEDLight Emitting Diode
PCPersonal Computer
SOPStandard Operating Procedure
cmCentimeter

7.0 ANNEXURES:
 Annex. No.                      Title
01 Calibration of Fourier Transform Infra-Red Spectrophotometer

8.0 SOP REFERENCES
  • British Pharmacopoeia

END OF THE SOP


ANNEXURES :

Annex. No. 01  Calibration of Fourier Transform Infra-Red Spectrophotometer