FTIR Calibration SOP | Calibration of FTIR | IR Calibration | Calibration of Infrared Spectrophotometer |
- FTIR Calibration SOP covers below points:
- Precautions
- Calibration Parameters of FTIR
- Verification of Wave Number Scale
- Control of Resolution Performance
- FTIR Calibration format
1.0 OBJECTIVE :
- To provide a procedure for Calibration of Fourier Transform Infra-Red (FTIR) Spectrophotometer.
2.0 SCOPE :
- This procedure is applicable to FTIR Spectrophotometer
3.0 RESPONSIBILITY :
- Quality Control Chemist and above
4.0 ACCOUNTABILITY:
- Quality Control Head
5.0 PROCEDURE :
5.1 PRECAUTIONS
- Place the instrument as far as possible away from any strong magnetic or electric field or any electrical appliances generating a high frequency.
- Avoid placing it, where severe vibrations exist.
- Place the instrument free from dust and corrosive gases.
5.2 CALIBRATION
- Change the assembly for calibration instead of assembly for analysis for the calibration.
- Handle the calibration assembly very carefully.
- Handle the polystyrene film very gently and avoid direct contact to the film.
- Verification of Wave Number Scale
- Measure the spectrum of polystyrene film 4000 cm-1 to 650 cm-1 after the routine Background correction.
- Following bands should appear at the frequencies indicated below and should not deviate from the designated frequency by more than the tolerance indicated.
| Sr. No. | Wave-number (cm-1) | Acceptable Tolerance (cm-1) |
| 01. | 3060.0 ± 1.0 | 3059.0 –3061.0 |
| 02. | 2849.5 ± 1.0 | 2848.5 – 2850.5 |
| 03. | 1942.9 ± 1.0 | 1941.9 – 1943.9 |
| 04. | 1601.2 ± 1.0 | 1600.2 – 1602.2 |
| 05. | 1583.0 ± 1.0 | 1582.0 –1584.0 |
| 06. | 1154.5 ± 1.0 | 1153.5 – 1155.5 |
| 07. | 1028.3 ± 1.0 | 1027.3 – 1029.3 |
- Control of Resolution Performance
- Expand the recorded polystyrene spectrum in the region 3200 cm-1 to 2600 cm-1.
- Note down the absorption at wave numbers 2870 cm-1 (tolerance ± 1 cm-1) and 2849.5 cm-1 (tolerance ± 1 cm-1).
- The difference between the absorbances at the absorption minimum at 2870 cm-1 and the absorption maximum at 2849.5 cm-1 is greater than 0.33.
- Expand the polystyrene spectrum in the wave number region 1800 cm-1 to 1400 cm-1.
- Note down the absorption at wave numbers the difference the absorption at 1583 cm-1 (tolerance ± 1 cm-1) and 1589 cm-1 (tolerance ± 1 cm-1).
- The difference between the absorbances at the absorption minimum at 1589 cm-1 and the absorption maximum at 1583 cm-1 is greater than 0.08.
For General information on Infrared Absorption Spectroscopy click below link:
INFRARED ABSORPTION SPECTROSCOPY
6.0 ABBREVIATIONS:
| Abbreviation | Expanded form |
| FTIR | Fourier Transform Infra-Red |
| LED | Light Emitting Diode |
| PC | Personal Computer |
| SOP | Standard Operating Procedure |
| cm | Centimeter |
7.0 ANNEXURES:
| Annex. No. | Title |
| 01 | Calibration of Fourier Transform Infra-Red Spectrophotometer |
8.0 SOP REFERENCES
- British Pharmacopoeia
END OF THE SOP
ANNEXURES :
Annex. No. 01 Calibration of Fourier Transform Infra-Red Spectrophotometer
