Destruction of Microbial Waste-SOP

SOP for Destruction of Microbial Waste | Microbiological Waste Disposal SOP |

  • Microbiological Waste Disposal SOP covers below points :
    • Microbial Waste destruction procedure
    • Precautions
    • Destruction of microbial waste record format
1.0 OBJECTIVE :
  • To provide a written procedure for destruction of microbial waste.
2.0 SCOPE :
  • This procedure applies for destruction of microbial plate containing of media, microbial cultures.
3.0 RESPONSIBILITY :
  • Microbiologist
4.0 ACCOUNTABILITY:
  • Head of Department
5.0 PROCEDURE :
  • Wear nose mask and hand gloves prior to starting the destruction of microbial waste.
  • Collect and transfer all used microbiological media, culture and contaminated glassware to washing area.
  • Remove the liquid media and agar in a waste destruction container with the help of spatula.
  • Add a disinfection solution (Dettol or Savlon 2.5% v/v).
  • Leave the waste media and glassware for 30 minutes to facilitate reaction of disinfection solution.
  • Transfer all the media to S.S. container and keep the S.S. container to autoclave.
  • Start the autoclave at 15 lbs/in2 for 30 minute.
  • After completing the cycle through the autoclave, Remove the S.S. container and drain out all the waste in the wash basin, after that just insert a bit of Dettol or Savlon 2.5% v/v in wash basin for caution.
  • Precaution:
    • Wash the hands with soap and then rinse with 70% v/v IPA after completion the destruction operation.
    • Do not autoclave the fresh media for routine use with destruction cycle.
6.0 ABBREVIATIONS:
AbbreviationExpanded form
SOPStandard Operating Procedure
S.S.Stainless  Steel
IPAIso Propyl Alcohol
v/vVolume / Volume
%Percentage
°CDegree Celsius
lbs/in2Pound per Square Inch

7.0 ANNEXURES:
Annex. No.Title
01 Destruction of microbial waste

8.0 SOP REFERENCES:
  • In-House

END OF THE SOP


ANNEXURES :

Annex. No. 01 Destruction of microbial waste