SOP for incubator | Incubator Cleaning Procedure |
- SOP for incubator covers below points :
- Incubator Cleaning Procedure
- Incubator Operation Procedure
- Temperature Monitoring Record of Incubators format
- Cleaning Record of Incubator format
1.0 OBJECTIVE :
- To describe procedure for Cleaning and Operation of Incubators.
2.0 SCOPE :
- This procedure is applicable to Incubators.
3.0 RESPONSIBILITY :
- Microbiologist and Lab assistant under supervision of microbiologist
4.0 ACCOUNTABILITY:
- Head of Department
5.0 PROCEDURE :
5.1 Cleaning :
- Switch “OFF” the main power supply of the incubator.
- Disconnect the plug of the equipment.
- Remove all the plates and keep under LAF at the time of cleaning.
- Take out the trays from the incubator and place it in a safe place.
- Clean the trays by mopping with 70% v/v IPA.
- Clean the internal surface and outer surface by mopping with 70% v/v IPA with clean cloth.
- Clean one incubator at a time and maintain the record in format of “Cleaning Record of Incubator”
- Put back all the plates in incubator after completion of cleaning.
- Frequency: Twice in month
5.2 Operation:
- Ensure that the incubator is properly connected to the main power supply.
- Switch “ON” the main power supply.
- Set the desired temperature as described in setting parameter, if required.
- Open the main door and lead of incubator, keep media containing tubes and plates for incubation.
- Close the lead and door properly after completion of work.
- Monitor the temperature twice in day.
- Maintain Incubator Usage log book.
5.3 Setting of temperature:
- Expo-Hi Tech incubator
- Press the SET key the display flash the SP code.
- Set temperature by UP and DOWN key as per temperature range requirement.
- Exit from setting by again press SET key.
- CEW / PSI incubator
- Press the SET key display show SP1.
- Set temperature by UP and DOWN key.
- Press SET key to exit from setting and save the set temperature.
- In case of if Temperature shoot up, above the range follow the procedure as mentioned below.
- Press the UP and DOWN key at a time for 3 second.
- Display show Tune (NO/YES) it changes from NO to YES by UP key.
- Again press SET key to start Tuning, it takes more than 3 hours.
- During Tuning process the controller calibrates itself by increase or decrease the Temperature during tuning process.
- Do not press any KEY of controller during Tuning.
- Setting parameter of all incubators should be performed by using User Manual of Controller for precaution, after permission of the HEAD, if required only.
- Record the temperature of incubators daily (Twice in a day), in format of “Temperature Monitoring Record of Incubators”
- If the incubator set temperature goes out of limit then first check the inside temperature of incubator by inserting calibrated temperature thermometer, then check the temperature shown on temperature thermometer.
- If temperature thermometer shows the same as temperature controller shows then intimate to QC Head / Designee, Engineering Head/ Designee and Q.A. Head/ Designee, take the necessary action.
- Document the incidence for the same and transfer the material present in incubator to another stand by incubator.
6.0 ABBREVIATIONS:
| Abbreviation | Expanded form |
| % | Percentage |
| v/v | Volume/Volume |
| °C | Degree Celsius |
| LAF | Laminar Air Flow |
7.0 ANNEXURES:
| Annex. No. | Title |
| 01 | Temperature Monitoring Record of Incubators |
| 02 | Cleaning Record of Incubator |
8.0 SOP REFERENCES
- Users Manuals of Incubator
END OF THE SOP
ANNEXURES :
Annex. No. 01 Temperature Monitoring Record of Incubators
Annex. No. 02 Cleaning Record of Incubator
