Cleaning and operation of Laminar Air Flow (LAF)-SOP

SOP for Laminar Air Flow

1.0 OBJECTIVE :
  • To describe a procedure for Cleaning and Operation of Laminar Air Flow.
2.0 SCOPE :
  • This procedure is applicable to CLEAN AIR make standard model of LAF.
3.0 RESPONSIBILITY :
  • microbiologist
4.0 ACCOUNTABILITY:
  • Head of Department
5.0 PROCEDURE :
5.1 Cleaning :
  • Disconnect the instrument from electrical connections.
  • Clean the outer surface and working bench of LAF with 70 % v/v Iso Propyl Alcohol Solution.
5.2 Operation:
  • Switch “ON” the main supply.
  • Switch on the main switch of cabinet.
  • Switch on the air flow and check the LAF monometer pressure (Limit 10 to 20 mm of water gauge)
  • Switch on the UV lamp half an hour before starting the work and also check UV burning Hours record.
  • The burning hour of UV light must not cross 90% of burning hour as claimed by Manufacturer.
  • Switch off the UV lamp after 30 minutes.
  • Switch on the visible light.
  • Clean the bench before starting microbial analysis with 70% v/v Iso Propyl Alcohol.
  • Carry out necessary work.
  • After completion of work, switch “OFF” the LAF, and main switch.
6.0 ABBREVIATIONS:
AbbreviationExpanded form
SOPStandard Operating Procedure
%Percentage
v/vVolume/Volume
mmMillimeter
UVUltraviolet
LAFLaminar Air Flow

7.0 ANNEXURES:
Annex. No.Title
01LAF- UV Burning Hours Record

8.0 SOP REFERENCES
  • Users Manual

END OF THE SOP


ANNEXURES :

Annex. No. 01 LAF- UV Burning Hours Record