SOP for Laminar Air Flow
1.0 OBJECTIVE :
- To describe a procedure for Cleaning and Operation of Laminar Air Flow.
2.0 SCOPE :
- This procedure is applicable to CLEAN AIR make standard model of LAF.
3.0 RESPONSIBILITY :
- microbiologist
4.0 ACCOUNTABILITY:
- Head of Department
5.0 PROCEDURE :
5.1 Cleaning :
- Disconnect the instrument from electrical connections.
- Clean the outer surface and working bench of LAF with 70 % v/v Iso Propyl Alcohol Solution.
5.2 Operation:
- Switch “ON” the main supply.
- Switch on the main switch of cabinet.
- Switch on the air flow and check the LAF monometer pressure (Limit 10 to 20 mm of water gauge)
- Switch on the UV lamp half an hour before starting the work and also check UV burning Hours record.
- The burning hour of UV light must not cross 90% of burning hour as claimed by Manufacturer.
- Switch off the UV lamp after 30 minutes.
- Switch on the visible light.
- Clean the bench before starting microbial analysis with 70% v/v Iso Propyl Alcohol.
- Carry out necessary work.
- After completion of work, switch “OFF” the LAF, and main switch.
6.0 ABBREVIATIONS:
| Abbreviation | Expanded form |
| SOP | Standard Operating Procedure |
| % | Percentage |
| v/v | Volume/Volume |
| mm | Millimeter |
| UV | Ultraviolet |
| LAF | Laminar Air Flow |
7.0 ANNEXURES:
| Annex. No. | Title |
| 01 | LAF- UV Burning Hours Record |
8.0 SOP REFERENCES
- Users Manual
END OF THE SOP
ANNEXURES :
Annex. No. 01 LAF- UV Burning Hours Record
