Antibiotic zone reader SOP
1.0 OBJECTIVE :
- To provide a procedure for Cleaning & Operation of Zone reader.
2.0 SCOPE :
- This procedure is applicable to EXPO HI-TECH make Zone Reader.
3.0 RESPONSIBILITY :
- Lab assistant under supervision of microbiologist & microbiologist
4.0 ACCOUNTABILITY:
- Head of Department
5.0 PROCEDURE :
5.1 Cleaning :
- Disconnect the instrument from electrical connection.
- Clean the outer surface using swab of 70% v/v Iso Propyl Alcohol.
5.2 Operation:
- Connect the instrument to the main power supply.
- Switch “ON” the Lamp.
- Set the vernier scale to zero – zero coincidence.
- Place the microbiological assay plate and measure accurately the zone of inhibition or exhibition.
- Keep the plate on the stage.
- Adjust the plate such that one side of circumference of the zone touches the marked line of the view glass.
- Turn the knob in clockwise direction so that the plate move across the marked line of the view glass until the opposite edge of the zone is in tangent to the marked line of the view glass.
- The scale reading gives nearest 0.2 mm on the rotating scale.
- Switch “OFF” the mains after completion of work.
6.0 ABBREVIATIONS:
| Abbreviation | Expanded form |
| SOP | Standard Operating Procedure |
| mm | Millimeter |
| % | Percentage |
| v/v | Volume/Volume |
7.0 ANNEXURES:
| Annex. No. | Title |
| 01 | Cleaning Record of Zone Reader |
8.0 SOP REFERENCES
- Users Manual
END OF THE SOP
ANNEXURES :
Annex. No. 01 Cleaning Record of Zone Reader
