Cleaning and operation of light microscope-SOP

SOP for Microscope

1.0 OBJECTIVE :
  • To provide a procedure for Cleaning and Operation of  light Microscope.
2.0 SCOPE :
  • This procedure is applicable to Microscope (Standard Model Labomed Vision 2000).
3.0 RESPONSIBILITY :
  • Lab assistant under supervision of microbiologist & microbiologist
4.0 ACCOUNTABILITY :
  • Quality Control Head
5.0 PROCEDURE :
5.1 Cleaning :
  • Disconnect the instrument from electrical connection.
  • Clean the instrument with soft cloth.
  • Clean the eyepiece, objective and optical parts (mirror, lens) using soft cloth or tissue paper.  
  • Also clean with  Cotton dipped in acetone and dry in air.
5.2 Operation :
  • Switch on the main switch of microscope.
  • Adjust the high power objective, by rotating the revolving nose piece in position.
  • Position the slide to bring into the exact center of the light source.
  • Focus high magnification objective lens on the center of the sample.
  • Turn the coarse adjustment knob to adjust the stage.
  • Bring the object into focus by adjusting with the coarse Knob. Focus the sample for better resolution by using fine adjustment knob.
  • Adjust the diaphragm level to decrease or increase the light intensity to produce the clear and sharp image.
  • After completion of observation, remove the slide and clean the objective lens  with cotton soaked in xylene.  
  • Switch off the main switch of microscope.
6.0 ABBREVIATIONS :

Nil

7.0 ANNEXURES :

Nil

8.0 SOP REFERENCES :
  • Users Manual

END OF THE SOP