SOP for Colony Counter
1.0 OBJECTIVE :
- To provide a procedure for Cleaning and Operation of Colony Counter.
2.0 SCOPE :
- This procedure is applicable to count the number of colonies in Microbiology Section using DBK make Instruments.
3.0 RESPONSIBILITY :
- Lab assistant under supervision of microbiologist & microbiologist
4.0 ACCOUNTABILITY:
- Head of Department
5.0 PROCEDURE :
5.1 Cleaning :
- De-dust the external surface of colony counter and magnifier with lint free duster.
- Clean the external surface of colony counter with 70 % v/v Iso Propyl Alcohol.
- Frequency: Daily
5.2 Operation:
- Connect the colony counter to the main supply and switch “ON” the power switch.
- Switch “ON” the mains switch of the equipment.
- Digital display show “0000”, if the digital display is not showing “0000” then press the reset button, digital display show “0000”.
- Keep the petri dish on illuminating surface.
- Adjust the magnifying glass to view clear colonies present on the petri dish.
- Press the tip of the pen on the colonies present on the petri dish and count the number of colonies.
- Record the number of colonies.
- Remove the plate from the wolffhuegel grid plate.
- Press the reset button.
- Switch off the mains after completion of work.
6.0 ABBREVIATIONS:
| Abbreviation | Expanded form |
| SOP | Standard Operating Procedure |
| % | Percentage |
| v/v | Volume/Volume |
7.0 ANNEXURES:
| Annex. No. | Title |
| 01 | Cleaning Record of Colony Counter |
8.0 SOP REFERENCES
- Users Manual
END OF THE SOP
ANNEXURES :
Annex. No. 01 Cleaning Record of Colony Counter
